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Binding to HLA-C*06:02 was verified using the NetMHCpan - 4.1 server ( Generation of plasmid-encoded peptides for V3S1/V13S1-TCR hybridoma stimulation For expression of short candidate peptides, forward and reverse oligonucleotides (Biomers) carrying a 5-CACCATG overhang and a stop codon in 3-position of the target sequence ( Supplementary Table S2 ) were annealed and ligated into pcDNA3.1D/V5-His-TOPO using pcDNA3.1 Directional TOPO Expression Kit (Invitrogen, Carlsbad, USA) according to manufacturers instructions as described (13, 14)

Targeting mitochondrial dysfunction and reactive oxygen species for neurodegenerative disease treatment

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RNA targeting therapeutics: molecular mechanisms of antisense oligonucleotides as a therapeutic platform